I studied on the absorption curves, on the effect of concentration of reductants and reaction time, on the linearity in the determination of inorganic phosphorus, on the effect of ammonium molybdate in the presence of different reductants used in laboratory for the determination of inorganic phosphorus.
The result were as follows;
1. All the methods were shown the highest absorbance at 650nm-800nm, approximately.
2. On the effect of the concentrator of reductants and the reaction time on the results of reaction., The results of reaction were increased more and more by increasing of the concentration of reductants (0.10.5)g~c and the reaction time(10-80min. ). The optimal reaction time was 10 minutes and the reaction was finished almost for 30 minutes. The optimal concentration of reductants in reaction was 0.2g%.
3. On the linearity, When the reaction was carried out at the concentration of 0.2g%of redu ctants. respectively, The linearity was occured at the concentration of 0.05rrig~0 to 3mgC (0.5 in serum) of inorganic phosphorus (Ascorbic acid), 0.05-2mgc~ (0.5,20mg% in sera;_) (Phenylhydrazine, 1, 2, 4- A minonaphthol sulionic acid, An.idol), and 0.05mgco to lmg% (0.5- 1;lmg% in serum) (Ferrous sulfate).
4. U- the effect of ammonium molybdate concentration on the reaction in the presence of red::ctant (0.2g%), The reaction in the amidol was finished almost at the concentration of 4g` ammo nium molybdate and the reaction in the other´s reductant was finished at the co ncen_ration of 3g ammonium molybdate, but the reaction was occured weakly at the concentration of lgO/o ammonium molybdate.
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